Mahuta ♥ Posted March 25, 2011 Report Share Posted March 25, 2011 The dimples will have drops of iodine solution, so that they'll be ready when you are testing the solution in the test tube every 30 seconds. Reply Link to post Share on other sites More sharing options...
Markee Posted March 25, 2011 Author Report Share Posted March 25, 2011 Yeah, that's what I was saying a while back 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 25, 2011 Report Share Posted March 25, 2011 Yeah okay, haha.So tell me how it goes Reply Link to post Share on other sites More sharing options...
Markee Posted March 25, 2011 Author Report Share Posted March 25, 2011 . . .Tell you how it goes? Meaning, i'm going to explain it to you how I exactly should do it? Or the lab work afterwards? 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 25, 2011 Report Share Posted March 25, 2011 LOl, after you do it..tell me if it went well, as in..everything worked out fine. Reply Link to post Share on other sites More sharing options...
Markee Posted March 25, 2011 Author Report Share Posted March 25, 2011 AHH! Ok ok I sure will. As long as I don't get amylase all over my face XDD 1 Reply Link to post Share on other sites More sharing options...
Markee Posted March 26, 2011 Author Report Share Posted March 26, 2011 The dimples will have drops of iodine solution, so that they'll be ready when you are testing the solution in the test tube every 30 seconds.How many drops btw? So my method would be, for each dimple, place a drop of iodine solution, afterwards, add the solution from each test tube to the iodine solution (or maybe 1 at a time - hehe) and time it's reaction? 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 26, 2011 Report Share Posted March 26, 2011 Yeah, so basically:2-3 drops of iodine into each dimple.Start with 100%starch solution, add the buffer and the moment you add the enzyme start timing. After 30 seconds take a small amount from the tube and drop it into the first dimple..then after the next 30 seconds, take another sample and drop it into the second dimple. Keep doing that until the color stops changing. Record the time of when it stopped.Then for to 75% starch solution and do the same..etc. Reply Link to post Share on other sites More sharing options...
Keel Posted March 26, 2011 Report Share Posted March 26, 2011 Im not sure why you would need to take samples from the test tube. The rate is basically time taken till colour change. By taking way samples you are tampering with the experiment. Reply Link to post Share on other sites More sharing options...
Markee Posted March 26, 2011 Author Report Share Posted March 26, 2011 Im not sure why you would need to take samples from the test tube. The rate is basically time taken till colour change. By taking way samples you are tampering with the experiment.EPIC AWESOMENESS! I meant to say by, how many drops will i be taking from the solution in the test tube. JAMES! Are you just doing this on purpose?! 1 Reply Link to post Share on other sites More sharing options...
Keel Posted March 26, 2011 Report Share Posted March 26, 2011 I am saying there is no need to take ANY drops from the test tube . if my calculations of correct my post will be on the NEW 4th page .Edit: damn, it wasnt Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 26, 2011 Report Share Posted March 26, 2011 How then? adding iodine to the test tube and see how long it takes for the color to stop changing? That doesn't make sense..if it changed to blue-black, it's not going to change back to brown. Reply Link to post Share on other sites More sharing options...
Markee Posted March 26, 2011 Author Report Share Posted March 26, 2011 HA! It didn't James! - And and, aaagh! Imagine this, using that sucking thing to get the solution from the test tube, once i've done that, how many drops of that goes into the iodine which is on the dimple? 1 Reply Link to post Share on other sites More sharing options...
Keel Posted March 26, 2011 Report Share Posted March 26, 2011 How then? adding iodine to the test tube and see how long it takes for the color to stop changing? That doesn't make sense..if it changed to blue-black, it's not going to change back to brown.Yes it can, when the iodine is placed into the starch solution it will turn blue-black due to the amylose long chains. When starch is broken up to individual glucose molecules the structure is destroyed and the colour of the solution will return back to the colour of iodine solution. Reply Link to post Share on other sites More sharing options...
Markee Posted March 26, 2011 Author Report Share Posted March 26, 2011 How then? adding iodine to the test tube and see how long it takes for the color to stop changing? That doesn't make sense..if it changed to blue-black, it's not going to change back to brown.Yes it can, when the iodine is placed into the starch solution it will turn blue-black due to the amylose long chains. When starch is broken up to individual glucose molecules the structure is destroyed and the colour of the solution will return back to the colour of iodine solution.I know that! I was referring to your post being on the 4th page of this thread! 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 26, 2011 Report Share Posted March 26, 2011 I just checked my biochem module and yes you're right, that was totally off my mind. Thanks I wonder why our teacher didnt tell us to use that way, aaand why do all the website mention that method? WeirdThen Mark, doing it all in one tube is much easier. Reply Link to post Share on other sites More sharing options...
Markee Posted March 26, 2011 Author Report Share Posted March 26, 2011 I just checked my biochem module and yes you're right, that was totally off my mind. Thanks I wonder why our teacher didnt tell us to use that way, aaand why do all the website mention that method? WeirdThen Mark, doing it all in one tube is much easier.Ahh, so I won't need the dimple trays then? 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 26, 2011 Report Share Posted March 26, 2011 Since this method works, no you don't. Reply Link to post Share on other sites More sharing options...
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