Keel Posted March 21, 2011 Report Share Posted March 21, 2011 Keel, you know when you mentioned Amylase? And that it breaks down starch with iodine solution. Then that would mean, I would not use a lipid(butter) as my substrate concentration?Well, if you use a different enzyme you obviously use a different substrate. Every enzyme's function is different. Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 21, 2011 Report Share Posted March 21, 2011 Mark, iodine solution tests for starch. It doesn't help amylase digest starch.Lipase-->LipidsAmylase---->StarchProtease(Trypsin, Pepsin..etc)-----> Proteins and polypeptides.Always keep that in mind. Reply Link to post Share on other sites More sharing options...
Markee Posted March 21, 2011 Author Report Share Posted March 21, 2011 YES Ma'am! Thanks Maha! I shall. . . Grrr, I seriously can not move on with my Bio SL coursework at this rate.. . 1 Reply Link to post Share on other sites More sharing options...
Markee Posted March 22, 2011 Author Report Share Posted March 22, 2011 Yo Guys! I can change the ENZYME!!!! WOOYEAH! So I'm picking Amylase 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 22, 2011 Report Share Posted March 22, 2011 Alright, you're using Amylase, now you have to write down:1)Substrate2)pH3)RQ4)CV5)IV6)DV Reply Link to post Share on other sites More sharing options...
Markee Posted March 22, 2011 Author Report Share Posted March 22, 2011 Independent Variable – PotatoDependent Variable – Activity of the Enzyme’s Reaction. Controlled Variable – Time -Concentration of ButterBasically : How can Substrate Concentration affect the activity of Amylase? 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 22, 2011 Report Share Posted March 22, 2011 No..no..no..no..no Independent Variable – Potato (you have to CONCENTRATION of X)Dependent Variable – Activity of the Enzyme’s Reaction. Controlled VariableS – Time, Amount of enzyme added, Amount of buffer solution, Temperature, Interval between testing..etc..etc anything you can think of.-Concentration of Butter (Butter has got nothing to do with this! It's fat)Basically : How can does Substrate Concentration affect the activity of Amylase? Reply Link to post Share on other sites More sharing options...
Markee Posted March 22, 2011 Author Report Share Posted March 22, 2011 oh wow. that was a lot Sorry Maha I won't put your efforts and the rest in vain. I will try to get the best mark for this design! 1 Reply Link to post Share on other sites More sharing options...
Keel Posted March 22, 2011 Report Share Posted March 22, 2011 Just to add to the control variables: Heavy metal concentration (0), inhibitors (0). These also affect the enzyme activity and need to be controlled too. Reply Link to post Share on other sites More sharing options...
Keel Posted March 23, 2011 Report Share Posted March 23, 2011 RQ: How does the change in concentration of starch affect the activity of amylase?Independent Variable: Concentration of Starch - varied from xg per dm3 to yg per dm3Dependent Variable: Time taken for solution to turn Benedict's solution from blue to brick red, OR time taken for solution to turn iodine solution black to red.Control Variables: Concentration and Volume of Amylase, Volume of Starch, Temperature (room), pH of solution (buffer), Concentration and Volume of Inhibitors (0 g per dm3), Concentration and Volume of heavy metals (0 g per dm3), volume of Benedict's solution/iodine solution. Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 23, 2011 Report Share Posted March 23, 2011 Yes Mark, what James said is right. I would like to add "time before testings", for example testing every 30 seconds is not testing every minute. Reply Link to post Share on other sites More sharing options...
Markee Posted March 25, 2011 Author Report Share Posted March 25, 2011 (edited) "Time before testings"? How does that work? So basically with this experiment, I would be using a Potato and the amylase+iodine solution mix em up whilst timing how long does it take for the color to change which should indicate the reaction is taking and has taken place?(This thread will not reach 10 pages! ) Edited March 25, 2011 by Mark10B 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 25, 2011 Report Share Posted March 25, 2011 Errrmm...didn't really get that, but:Why are you using potatoes? Just use starch solution! You are changing concentration, how are you going to change concentration of starch with potatoes?Use starch solution and make 4 or 5 different concentrations. As for testing:I forgot what a thing is called so here's the apparatus you must have:The red in the tiles are drops of iodine solution, you are going to have tubes with the solution, buffer and enzyme. Every 30 seconds or so you are going to take two drops using the dropping pipette and drop it on to one tile. When it stops changing color..all the starch is digested. Reply Link to post Share on other sites More sharing options...
Markee Posted March 25, 2011 Author Report Share Posted March 25, 2011 OH! I've done this before in a lab work in Biology Apparently it is kind of confusing doing this alone.lolol...ok. So it seems rather simple after all. Is there a site wherein it shows the procedure of doing this? 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 25, 2011 Report Share Posted March 25, 2011 No! Even if there is I am not going to tell you! I basically gave you the procedure try writing it down, it's really easy. Reply Link to post Share on other sites More sharing options...
Markee Posted March 25, 2011 Author Report Share Posted March 25, 2011 Oh, so basically, all I have to do is put each starch with different concentrations on a vacant dimple on the tray, and then mix a drop of amylase with the iodine solution and time the reaction when it the color changes? 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 25, 2011 Report Share Posted March 25, 2011 No,lol..read what I said again, the dimples will have the iodine solution. The starch+buffer+enzyme will go in the test tube. Reply Link to post Share on other sites More sharing options...
Markee Posted March 25, 2011 Author Report Share Posted March 25, 2011 So...I fill out the trays with the different concentrations of the iodine solution, THEN, I add the solutions in the test tube, and time the reaction or when it changes color? 1 Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted March 25, 2011 Report Share Posted March 25, 2011 Lol, no..you are going to change the concentration of starch solution, not iodine.Here it is:TUBE 1: 100% starch + buffer solution + amylase.TUBE 2: 75% starch + buffer solution + amylase.etcetcetc.You are going to do one tube at a time..take a sample every 30 seconds and drop it onto a dimple, keep doing that until the color stops changing. Reply Link to post Share on other sites More sharing options...
Markee Posted March 25, 2011 Author Report Share Posted March 25, 2011 (edited) oh, yeah, the other way around. . .So, nothing will be on the dimple then? =/I am asking as to what will be on the dimple tray before I add the solutions from the tubes. Edited March 25, 2011 by Mark10B 1 Reply Link to post Share on other sites More sharing options...
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