Raptors fan Posted March 8, 2017 Report Share Posted March 8, 2017 Hi all, so I used a uv-vis spec for my lab but for some reason, my graph got deleted but i still have my data from the spectrometer. Is there a way that i can create a concentration vs absorbance graph without having the spectrometer inserted? THANK YOU Reply Link to post Share on other sites More sharing options...
kw0573 Posted March 9, 2017 Report Share Posted March 9, 2017 You would be just plotting absorbance for selected wavelengths from your data against concentration, which you can do manually. The key would be to have a program that can interpret the data file type, for example .csv (comma separated value) can be opened by MS Excel. If not already, you should always ensure that the file formats can be accessible without the original probe/instrument. Good luck! Reply Link to post Share on other sites More sharing options...
Raptors fan Posted March 9, 2017 Author Report Share Posted March 9, 2017 Thanks for replying. How would I select wavelengths? Reply Link to post Share on other sites More sharing options...
kw0573 Posted March 9, 2017 Report Share Posted March 9, 2017 Just now, Raptors fan said: Thanks for replying. How would I select wavelengths? In my understanding, UV-VIS spectroscopy selects a range of wavelengths and find the absorption for each. If you can access the data, it should be tabulated by different wavelengths (eg as a header). You would pick just the absorption for the wavelengths you want. So for example if you made 6 solutions, there would be 6 data points for each wavelength on the absorbance / concentration graph. You should probably look up a tutorial for your specific data format/software, but it's definitely doable. Reply Link to post Share on other sites More sharing options...
Raptors fan Posted March 9, 2017 Author Report Share Posted March 9, 2017 thank you so much :), is there any other calculations that i could include? what about beer's law Reply Link to post Share on other sites More sharing options...
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