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Enzyme Lab Help!


Spacecat

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Hi!

 

I am doing my IA lab, and just wanted to ask opinions regarding my lab. Is it acceptable to refer to solutions as: 10% cornstarch solution (for example: 10 grams cornstarch and 100 ml water) or should I convert this to moles (so complicated tho - not chem student)? Second, my quantitative data will mostly be colour absorbance (result from benedicts test). How does one interpret these quantitatively in terms of glucose concentration?

 

Thanks for any help!! :D

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The Beer-Lambert's Law relates absorbance and molar concentration. The concentration here is for the coloured complex. Google says copper (II) sulfate is the coloured complex so you should figure out the molar concentration of copper (II) ions and the molar absoptivity of copper (II) sulfate. 

You should ask your biology teacher to help you with the calculations. 

I assume that other than for such calculations, you can use concentrations by mass. 

Edited by kw0573
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