Blee711 Posted October 10, 2012 Report Share Posted October 10, 2012 Okay, so for my Biology HL labs I'm having a bit of trouble. For my experiment, I am testing the effects of temperature on Lipase. To do this I am combining vegetable oil (because of the lipids) and Lipase in a test tube - in a water bath- and using Sudan III as my indicator. The major obstacle I am facing currently is figuring out the proportions of substances. How much vegetable oil to use with how much Lipase?Any helps/tips? Thanks! Reply Link to post Share on other sites More sharing options...
Mahuta ♥ Posted October 10, 2012 Report Share Posted October 10, 2012 I don't think there are specific amounts you need to use. You can use for example, 25 ml of oil and 5 ml of lipase. You could do a test one, try those proportions and see if you can get some reasonable results, if the reaction is way too strong or not strong enough you can just adjust it. Reply Link to post Share on other sites More sharing options...
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