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Bio Labs- Indicator help? (Sudan III)


Blee711

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Okay, so for my Biology HL labs I'm having a bit of trouble. For my experiment, I am testing the effects of temperature on Lipase. To do this I am combining vegetable oil (because of the lipids) and Lipase in a test tube - in a water bath- and using Sudan III as my indicator.

The major obstacle I am facing currently is figuring when I put the 3 drops of Sudan III in the test tube after I have allowed 5 mins for the reaction to occur, I mix the indicator in and all that happens is the mixture because a shade darker.

Am I not suppose to mix the indicator in?

How do I get data from this?

Any helps/tips?

Thanks!

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