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Enzyme IA lab help?


JaeJ

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Hello~

I'm planning to use different concentrations of lipase on cream milk, and then measure the pH (which it's supposed to get more acidic), but I'm not sure what concentrations I should use.

Has anyone tried this or a similar lab before? at school we can't do a preliminary test (we have just one try) because of the limited resources, and I don't want to ruin it, so any suggestions would be appreciated, also related to the time I should leave them react .

Thanks (excuse my bad english) :]

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What are you trying to measure? Changing the concentration is going to alter the rate of the reaction going up to the point where you saturate the milk with lipase. I would use very low concentrations. Probably less than 1M would work and then leave it for anywhere from 5-10min. Keep the time constant of course.

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